Comparative Evaluation of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Concentrating On Nucleic Acid Extraction.

(LNJNbio Polystyrene Microspheres)

In the area of modern-day biotechnology, microsphere materials are extensively used in the removal and filtration of DNA and RNA because of their high certain area, great chemical security and functionalized surface homes. Among them, polystyrene (PS) microspheres and their derived polystyrene carboxyl (CPS) microspheres are among the two most widely examined and used materials. This post is supplied with technological assistance and information evaluation by Shanghai Lingjun Biotechnology Co., Ltd., intending to systematically compare the performance differences of these two sorts of products in the process of nucleic acid extraction, covering vital indicators such as their physicochemical homes, surface adjustment capability, binding efficiency and recuperation price, and highlight their appropriate scenarios with speculative data.

Polystyrene microspheres are uniform polymer particles polymerized from styrene monomers with excellent thermal stability and mechanical stamina. Its surface area is a non-polar framework and usually does not have energetic practical teams. As a result, when it is straight utilized for nucleic acid binding, it requires to count on electrostatic adsorption or hydrophobic activity for molecular addiction. Polystyrene carboxyl microspheres present carboxyl useful groups (– COOH) on the basis of PS microspheres, making their surface area capable of more chemical coupling. These carboxyl teams can be covalently bonded to nucleic acid probes, proteins or other ligands with amino groups with activation systems such as EDC/NHS, consequently achieving much more steady molecular fixation. Therefore, from an architectural viewpoint, CPS microspheres have a lot more advantages in functionalization capacity.

Nucleic acid removal typically consists of actions such as cell lysis, nucleic acid launch, nucleic acid binding to solid phase service providers, cleaning to eliminate impurities and eluting target nucleic acids. In this system, microspheres play a core function as strong phase service providers. PS microspheres primarily depend on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding effectiveness has to do with 60 ~ 70%, yet the elution performance is low, only 40 ~ 50%. In contrast, CPS microspheres can not only make use of electrostatic results yet likewise achieve even more solid fixation through covalent bonding, reducing the loss of nucleic acids throughout the cleaning procedure. Its binding effectiveness can reach 85 ~ 95%, and the elution efficiency is additionally enhanced to 70 ~ 80%. In addition, CPS microspheres are likewise significantly better than PS microspheres in regards to anti-interference ability and reusability.

In order to verify the performance distinctions in between the two microspheres in real operation, Shanghai Lingjun Biotechnology Co., Ltd. conducted RNA removal experiments. The experimental examples were derived from HEK293 cells. After pretreatment with conventional Tris-HCl buffer and proteinase K, 5 mg/mL PS and CPS microspheres were made use of for extraction. The outcomes showed that the ordinary RNA yield extracted by PS microspheres was 85 ng/ μL, the A260/A280 proportion was 1.82, and the RIN value was 7.2, while the RNA yield of CPS microspheres was raised to 132 ng/ μL, the A260/A280 proportion was close to the ideal worth of 1.91, and the RIN worth got to 8.1. Although the operation time of CPS microspheres is somewhat longer (28 mins vs. 25 mins) and the cost is greater (28 yuan vs. 18 yuan/time), its extraction high quality is significantly enhanced, and it is more suitable for high-sensitivity detection, such as qPCR and RNA-seq.

( SEM of LNJNbio Polystyrene Microspheres)

From the point of view of application scenarios, PS microspheres appropriate for massive screening tasks and initial enrichment with low requirements for binding specificity because of their low cost and basic procedure. However, their nucleic acid binding capability is weak and conveniently influenced by salt ion focus, making them unsuitable for long-term storage or repeated use. In contrast, CPS microspheres appropriate for trace sample extraction as a result of their rich surface useful teams, which facilitate more functionalization and can be utilized to create magnetic bead detection packages and automated nucleic acid removal platforms. Although its prep work procedure is fairly complicated and the expense is fairly high, it shows stronger versatility in clinical research study and medical applications with rigorous requirements on nucleic acid removal performance and purity.

With the quick advancement of molecular diagnosis, genetics editing, liquid biopsy and various other fields, greater demands are placed on the performance, pureness and automation of nucleic acid extraction. Polystyrene carboxyl microspheres are progressively changing traditional PS microspheres due to their outstanding binding performance and functionalizable qualities, ending up being the core choice of a new generation of nucleic acid extraction materials. Shanghai Lingjun Biotechnology Co., Ltd. is also constantly optimizing the bit dimension circulation, surface area density and functionalization performance of CPS microspheres and developing matching magnetic composite microsphere products to meet the needs of clinical diagnosis, scientific study organizations and commercial consumers for top quality nucleic acid removal services.

Vendor

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need polystyrene microspheres carboxyl, please feel free to contact us at sales01@lingjunbio.com.

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